Plasmid_Backbone

Part:BBa_K2429061:Design

Designed by: Nia Myrie   Group: iGEM17_MIT   (2017-10-25)


pDEST mCherry vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2508
    Illegal PstI site found at 2353
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal PstI site found at 2353
    Illegal NotI site found at 1393
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 1464
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2508
    Illegal PstI site found at 2353
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2508
    Illegal PstI site found at 2353
    Illegal NgoMIV site found at 3071
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 212


Design Notes

Source

This is an updated version of a previous part. The mCherry gene comes from a species of coral

References